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	Provedor de dados BABT (1) BJMBR (1) Autor Latha,M. (1) Ma,Chunmei (1) Pari,L. (1) Sun,Zhen (1) Zhou,Jie (1) Zhu,Shuhua (1) Mais... Palavra-chave Sorbitol dehydrogenase (2) Diabetes (1) Enzyme assay (1) Expression and purification (1) Glutathione peroxidase (1) Glutathione-S-transferase (1) Insulin (1) Lipid peroxidation (1) Ni-NTA (1) Peach fruit (1) Recombinant SDH (1) Scoparia dulcis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 2004 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Cloning, expression, purification and assay of sorbitol dehydrogenase from "Feicheng" peach fruit (Prunus persica) BABT Sun,Zhen; Ma,Chunmei; Zhou,Jie; Zhu,Shuhua. A gene encoding NAD+-dependent sorbitol dehydrogenase (SDH) in peach fruit was cloned and expressed in Escherichia coli. Recombinant SDH protein with 6×His-tagged was localized exclusively in the cytoplasmic soluble fraction of E. coli when the strains were grown for 4-5 h at 37 ºC. Highly pure protein was isolated by Ni2+-resin chromatography with 150 mM imidazole in 50 mM Tris, pH 8.0, by elution. In order to ensure that the recombinant SDH could be used for further study, the fluorescence and ultraviolet spectrum of the recombinant SDH were detected. Recombinant SDH was confirmed to be capable of oxidizing sorbitol by enzymatic activity assay. The activity of the recombinant SDH was 2.73 U mg-1min-1, which was similar with that directly extracted from... Tipo: Info:eu-repo/semantics/article Palavras-chave: Sorbitol dehydrogenase; Recombinant SDH; Expression and purification; Ni-NTA; Enzyme assay; Peach fruit. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400002 Effect of an aqueous extract of Scoparia dulcis on blood glucose, plasma insulin and some polyol pathway enzymes in experimental rat diabetes BJMBR Latha,M.; Pari,L.. The effects of an aqueous extract of the plant Scoparia dulcis (200 mg/kg) on the polyol pathway and lipid peroxidation were examined in the liver of streptozotocin adult diabetic male albino Wistar rats. The diabetic control rats (N = 6) presented a significant increase in blood glucose, sorbitol dehydrogenase, glycosylated hemoglobin and lipid peroxidation markers such as thiobarbituric acid reactive substances (TBARS) and hydroperoxides, and a significant decrease in plasma insulin and antioxidant enzymes such as glutathione peroxidase (GPx), glutathione-S-transferase (GST) and reduced glutathione (GSH) compared to normal rats (N = 6). Scoparia dulcis plant extract (SPEt, 200 mg kg-1 day-1) and glibenclamide (600 µg kg-1 day-1), a reference drug, were... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glutathione peroxidase; Glutathione-S-transferase; Insulin; Lipid peroxidation; Scoparia dulcis; Sorbitol dehydrogenase; Diabetes. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000400015 Registros recuperados: 2 Primeira ... 1 ... Última

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